摘要Background:In rodents,research has revealed a role of liver X receptors(LXR)in controlling lipid homeostasis and regulating the synthesis of polyunsaturated fatty acids(PUFA).Recent data suggest that LXRB is the predominant LXR subtype in ruminant mammary cells,but its role in lipid metabolism is unknown.It was hypothesized that LXRB plays a role in lipid homeostasis via altering the synthesis of PUFA in the ruminant mammary gland.We used overexpres-sion and knockdown of LXRB in goat primary mammary epithelial cells(GMEC)to evaluate abundance of lipogenic enzymes,fatty acid profiles,content of lipid stores and activity of the stearoyl-CoA desaturase(SCD1)promoter.Results:Overexpression of LXRB markedly upregulated the protein abundance of LXRB while incubation with siRNA targeting LXRB markedly decreased abundance of LXRB protein.Overexpression of LXRB plusT0901317(T09,a ligand for LXR)dramatically upregulated SCD1 and elongation of very long chain fatty acid-like fatty acid elongases 5-7(ELOVL 5-7),which are related to PUFA synthesis.Compared with the control,cells overexpressing LXRB and stimu-lated withT09 had greater concentrations of C16:0,16:1,18:1n7,18:1n9 and C18:2 as well as desaturation and elonga-tion indices of C16:0.Furthermore,LXRB-overexpressing cells incubated withT09 had greater levels of triacylglycerol and cholesterol.Knockdown of LXRB in cells incubated with T09 led to downregulation of genes encoding elongases and desaturases.Knockdown of LXRB attenuated the increase in triacylglycerol and cholesterol that was induced byT09.In cells treated with dimethylsulfoxide,knockdown of LXRB increased the concentration of C16:0 at the expense of C18:0,while a significant decrease in C18:2 was observed in cells incubated with both siLXRB andT09.The abundance of sterol regulatory element binding transcription factor 1 precursor(pSREBP1)and its mature fragment(nSREBP1)was upregulated byT09,but not LXRB overexpression.In the cells cultured withT09,knockdown of LXRB downregulated the abundance for pSREBP1 and nSREBP1.Luciferase reporter assays revealed that the activities of wild type SCD1 promoter or fragment with SREBP1 response element(SRE)mutation were decreased markedly when LXRB was knocked down.Activity of the SCD1 promoter that was induced byT09 was blocked when the SRE muta-tion was introduced.Conclusion:The current study provides evidence of a physiological link between the LXRB and SREBP1 in the rumi-nant mammary cell.An important role was revealed for the LXRB-SREBP1 network in the synthesis of PUFA via the regulation of genes encoding elongases and desaturases.Thus,targeting this network might elicit broad effects on lipid homeostasis in ruminant mammary gland.