摘要Background In beef cattle,more than 50％of the energy input to produce a unit of beef is consumed by the female that produced the calf.Development of genomic tools to identify females with high genetic merit for reproductive function could increase the profitability and sustainability of beef production.Results Genome-wide association studies(GWAS)were performed using a single-step genomic best linear unbiased prediction approach on pregnancy outcome traits from a population of Angus-Brahman crossbred heifers.Further-more,a validation GWAS was performed using data from another farm.Heifers were genotyped with the Bovine GGP F250 array that contains 221,077 SNPs.In the discovery population,heifers were bred in winter breeding seasons involving a single round of timed artificial insemination(AI)followed by natural mating for 3 months.Two phenotypes were analyzed:pregnancy outcome to first-service AI(PAI;n=1,481)and pregnancy status at the end of the breed-ing season(PEBS;n=1,725).The heritability was estimated as 0.149 and 0.122 for PAI and PEBS,respectively.In the PAI model,one quantitative trait locus(QTL),located between 52.3 and 52.5 Mb on BTA7,explained about 3％of the genetic variation,in a region containing a cluster of y-protocadherin genes and SLC25A2.Other QTLs explaining between 0.5％and 1％of the genetic variation were found on BTA12 and 25.In the PEBS model,a large QTL on BTA7 was synonymous with the QTL for PAI,with minor QTLs located on BTA5,9,10,11,19,and 20.The validation popula-tion for pregnancy status at the end of the breeding season were Angus-Brahman crossbred heifers bred by natu-ral mating.In concordance with the discovery population,the large QTL on BTA7 and QTLs on BTA10 and 12 were identified.Conclusions In summary,QTLs and candidate SNPs identified were associated with pregnancy outcomes in beef heifers,including a large QTL associated with a group of protocadherin genes.Confirmation of these associations with larger populations could lead to the development of genomic predictions of reproductive function in beef cat-tle.Moreover,additional research is warranted to study the function of candidate genes associated with QTLs.