摘要Background Our previous study has reported that supplementation of oligosaccharide-based polymer enhances gut health and disease resistance of pigs infected with enterotoxigenic E.coli(ETEC)F18 in a manner similar to car-badox.The objective of this study was to investigate the impacts of oligosaccharide-based polymer or antibiotic on the host metabolic profiles and colon microbiota of weaned pigs experimentally infected with ETEC F18.Results Multivariate analysis highlighted the differences in the metabolic profiles of serum and colon digesta which were predominantly found between pigs supplemented with oligosaccharide-based polymer and antibiotic.The rela-tive abundance of metabolic markers of immune responses and nutrient metabolisms,such as amino acids and car-bohydrates,were significantly differentiated between the oligosaccharide-based polymer and antibiotic groups(q＜0.2 and fold change＞2.0).In addition,pigs in antibiotic had a reduced(P＜0.05)relative abundance of Lachno-spiraceae and Lactobacillaceae,whereas had greater(P＜0.05)Clostridiaceae and Streptococcaceae in the colon digesta on d 11 post-inoculation(PI)compared with d 5 PI.Conclusions The impact of oligosaccharide-based polymer on the metabolic and microbial profiles of pigs is not fully understood,and further exploration is needed.However,current research suggest that various mecha-nisms are involved in the enhanced disease resistance and performance in ETEC-challenged pigs by supplementing this polymer.