摘要Background Goose,descendants of migratory ancestors,have undergone extensive selective breeding,resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant energy intake.As a result,goose offers an excellent model for studying obesity,metabolic disorders,and liver diseases in mammals.Although the impact of the three-dimensional arrangement of chromatin within the cell nucleus on gene expression and transcriptional regulation is widely acknowledged,the precise functions of chromatin architecture reorganization during fat deposition in goose liver tissues still need to be fully comprehended.Results In this study,geese exhibited more pronounced changes in the liver index and triglyceride(TG)content following the consumption of the high-fat diet(HFD)than mice without significant signs of inflammation.Addition-ally,we performed comprehensive analyses on 10 goose liver tissues(5 HFD,5 normal),including generating high-resolution maps of chromatin architecture,conducting whole-genome gene expression profiling,and identifying H3K27ac peaks in the livers of geese and mice subjected to the HFD.Our results unveiled a multiscale restructuring of chromatin architecture,encompassing Compartment A/B,topologically associated domains,and interactions between promoters and enhancers.The dynamism of the three-dimensional genome architecture,prompted by the HFD,assumed a pivotal role in the transcriptional regulation of crucial genes.Furthermore,we identified genes that regulate chromatin conformation changes,contributing to the metabolic adaptation process of lipid deposition and hepatic fat changes in geese in response to excessive energy intake.Moreover,we conducted a cross-species analysis comparing geese and mice exposed to the HFD,revealing unique characteristics specific to the goose liver compared to a mouse.These chromatin conformation changes help elucidate the observed characteristics of fat deposition and hepatic fat regulation in geese under conditions of excessive energy intake.Conclusions We examined the dynamic modifications in three-dimensional chromatin architecture and gene expression induced by an HFD in goose liver tissues.We conducted a cross-species analysis comparing that of mice.Our results contribute significant insights into the chromatin architecture of goose liver tissues,offering a novel per-spective for investigating mammal liver diseases.