摘要Background Low crude protein(CP)formulations with supplemental amino acids(AA)are used to enhance intestinal health,reduce costs,minimize environmental impact,and maintain growth performance of pigs.However,extensive reduction of dietary CP can compromise growth performance due to limited synthesis of non-essential AA and limited availability of bioactive compounds from protein supplements even when AA requirements are met.Moreover,implementing a low CP formulation can increase the net energy(NE)content in feeds causing excessive fat deposition.Additional supplementation of functional AA,coupled with low CP formulation could further enhance intestinal health and glucose metabolism,improving nitrogen utilization,and growth performance.Three experi-ments were conducted to evaluate the effects of low CP formulations with supplemental AA on the intestinal health and growth performance of growing-finishing pigs.Methods In Exp.1,90 pigs(19.7±1.1 kg,45 barrows and 45 gilts)were assigned to 3 treatments:CON(18.0％CP,supplementing Lys,Met,and Thr),LCP(16.0％CP,supplementing Lys,Met,Thr,Trp,and Val),and LCPT(16.1％CP,LCP+0.05％SID Trp).In Exp.2,72 pigs(34.2±4.2 kg BW)were assigned to 3 treatments:CON(17.7％CP,meeting the requirements of Lys,Met,Thr,and Trp);LCP(15.0％CP,meeting Lys,Thr,Trp,Met,Val,Ile,and Phe);and VLCP(12.8％CP,meeting Lys,Thr,Trp,Met,Val,lie,Phe,His,and Leu).In Exp.3,72 pigs(54.1±5.9 kg BW)were assigned to 3 treat-ments and fed experimental diets for 3 phases(grower 2,finishing 1,and finishing 2).Treatments were CON(18.0％,13.8％,12.7％CP for 3 phases;meeting Lys,Met,Thr,and Trp);LCP(13.5％,11.4％,10.4％CP for 3 phases;meeting Lys,Thr,Trp,Met,Val,lle,and Phe);and LCPG(14.1％,12.8％,11.1％CP for 3 phases;LCP+Glu to match SID Glu with CON).All diets had 2.6 Mcal/kg NE.Results In Exp.1,overall,the growth performance did not differ among treatments.The LCPT increased(P＜0.05)Claudin-1 expression in the duodenum and jejunum.The LCP and LCPT increased(P＜0.05)CAT-1,4F2hc,and B0AT expressions in the jejunum.In Exp.2,overall,the VLCP reduced(P＜0.05)G∶F and BUN.The LCP and VLCP increased(P＜0.05)the backfat thickness(BFT).In Exp.3,overall,growth performance and BFT did not differ among treatments.The LCPG reduced(P＜0.05)BUN,whereas increased the insulin in plasma.The LCP and LCPG reduced(P＜0.05)the abundance of Streptococcaceae,whereas the LCP reduced(P＜0.05)Erysipelotrichaceae,and the alpha diversity.Conclusions When implementing low CP formulation,CP can be reduced by supplementation of Lys,Thr,Met,Trp,Val,and lie without affecting the growth performance of growing-finishing pigs when NE is adjusted to avoid increased fat deposition.Supplementation of Trp above the requirement or supplementation of Glu in low CP formu-lation seems to benefit intestinal health as well as improved nitrogen utilization and glucose metabolism.