摘要Background Adequate level of carbohydrates in aquafeeds help to conserve protein and reduce cost.However,studies have indicated that high-carbohydrate(HC)diet disrupt the homeostasis of the gut-liver axis in largemouth bass,resulting in decreased intestinal acetate and butyrate level.Method Herein,we had concepted a set of feeding experiment to assess the effects of dietary sodium acetate(SA)and sodium butyrate(SB)on liver health and the intestinal microbiota in largemouth bass fed an HC diet.The experi-mental design comprised 5 isonitrogenous and isolipidic diets,including LC(9％starch),HC(18％starch),HCSA(18％starch;2 g/kg SA),HCSB(18％starch;2 g/kg SB),and HCSASB(18％starch;1 g/kg SA+1 g/kg SB).Juvenile largemouth bass with an initial body weight of 7.00±0.20 g were fed on these diets for 56 d.Results We found that dietary SA and SB reduced hepatic triglyceride accumulation by activating autophagy(ATG101,LC3B and TFEB),promoting lipolysis(CPT1α,HSL and AMPKα),and inhibiting adipogenesis(FAS,ACCA,SCD1 and PPARγ).In addition,SA and SB decreased oxidative stress in the liver(CAT GPX1α and SOD1)by activating the Keap1-Nrf2 pathway.Meanwhile,SA and SB alleviated HC-induced inflammation by downregulating the expres-sion of pro-inflammatory factors(IL-1β,COX2 and Hepcidin1)through the NF-κB pathway.Importantly,SA and SB increased the abundance of bacteria that produced acetic acid and butyrate(Clostridium_sensu_stricto_1).Combined with the KEGG analysis,the results showed that SA and SB enriched carbohydrate metabolism and amino acid metab-olism pathways,thereby improving the utilization of carbohydrates.Pearson correlation analysis indicated that growth performance was closely related to hepatic lipid deposition,autophagy,antioxidant capacity,inflammation,and intes-tinal microbial composition.Conclusions In conclusion,dietary SA and SB can reduce hepatic lipid deposition;and alleviate oxidative stress and inflammation in largemouth bass fed on HC diet.These beneficial effects may be due to the altered composi-tion of the gut microbiota caused by SA and SB.The improvement effects of SB were stronger than those associated with SA.