摘要Background Sows commonly experience insulin resistance in late gestation and lactation,causing lower feed intake and milk production,which can lead to higher mortality rates in newborn piglets.The probiotic Lactobacillus rham-nosus GG(LGG)is known to improve insulin resistance.However,whether supplementing LGG can improve insulin sensitivity in sows and enhance lactation performance,particularly the early survival of offspring remains unclear.Hence,we explored the effects and mechanisms of supplementing LGG during late gestation and lactation on sow insulin sensitivity,lactation performance,and offspring survival.In total,20 sows were randomly allocated to an LGG(n=10)and control group(n=10).Results In sows,LGG supplementation significantly improved insulin sensitivity during late gestation and lactation,increased feed intake,milk production and colostrum lactose levels in early lactation,and enhanced newborn piglet survival.Moreover,LGG treatment significantly reshaped the gut microbiota in sows,notably increasing microbiota diversity and enriching the relative abundance of insulin sensitivity-associated probiotics such as Lactobacillus,Bifido-bacterium,and Bacteroides.Serum metabolite and amino acid profiling in late-gestation sows also revealed decreased branched-chain amino acid and kynurenine serum levels following LGG supplementation.Further analyses high-lighted a correlation between mitigated insulin resistance in late pregnancy and lactation by LGG and gut microbiota reshaping and changes in serum amino acid metabolism.Furthermore,maternal LGG enhanced immunity in new-born piglets,reduced inflammation,and facilitated the establishment of a gut microbiota.Conclusions We provide the first evidence that LGG mitigates insulin resistance in sows and enhances offspring survival by modulating the gut microbiota and amino acid metabolism.