摘要Background Liver lipid dysregulation is one of the major factors in the decline of production performance in late-stage laying hens.Silymarin(SIL),a natural flavonolignan extracted from milk thistle,is known for its hepatoprotective and lipid-lowering properties in humans.This study evaluates whether SIL can provide similar benefits to late-stage laying hens.A total of 480 68-week-old Lohmann Pink laying hens were randomly assigned into 5 groups,each group consisting of 6 replicates with 16 hens each.The birds received a basal diet either without silymarin(control)or supplemented with silymarin at concentrations of 250,500,750,or 1,000 mg/kg(SIL250,SIL500,SIL750,SIL1000)over a 12-week period.Results The CON group exhibited a significant decline in laying rates from weeks 9 to 12 compared to the initial 4 weeks(P=0.042),while SIL supplementation maintained consistent laying rates throughout the study(P＞0.05).Notably,the SIL500 and SIL750 groups showed higher average egg weight than the CON group during weeks 5 to 8(P=0.049).The SIL750 group had a significantly higher average daily feed intake across the study period(P＜0.05),and the SIL500 group saw a marked decrease in the feed-to-egg ratio from weeks 5 to 8(P=0.003).Furthermore,the SIL500 group demonstrated significant reductions in serum ALT and AST levels(P＜0.05)and a significant decrease in serum triglycerides and total cholesterol at week 12 with increasing doses of SIL(P＜0.05).SIL also posi-tively influenced liver enzyme expression(FASN,ACC,Apo-VLDL Ⅱ,FXR,and CYP7A1;P＜0.05)and altered the cecal microbiota composition,enhancing species linked to secondary bile acid synthesis.Targeted metabolomics identi-fied 9 metabolites predominantly involved in thiamin metabolism that were significantly different in the SIL groups(P＜0.05).Conclusions Our study demonstrated that dietary SIL supplementation could ameliorate egg production rate in late stage laying hens,mechanistically,this effect was via improving hepatic lipid metabolism and cecal microbiota function to achieve.Revealed the potentially of SIL as a feed supplementation to regulate hepatic lipid metabolism dysregulation.Overall,dietary 500 mg/kg SIL had the best effects.