摘要Background Deoxynivalenol(DON)is a mycotoxin that severely pollutes feed ingredients,and methods for reduc-ing DON toxicity have become a significant research direction.Chlorogenic acid(CGA)is an active polyphenol found in some plants,which has anti-inflammatory and antioxidant properties and a protective effect on animal intestinal health.The effects of CGA on DON-induced pyroptosis in the intestinal porcine epithelial cell line-J2(IPEC-J2)and its potential mechanism were explored in this study.Results IPEC-J2 cells viability and membrane integrity were inversely correlated with DON concentration.Compared to those in the group treated with DON alone at 2,500 ng/mL,pretreatment with 80 μmol/L CGA for 4 h significantly improved cell viability(P＜0.01),and the alleviation of typical pyroptotic symptoms induced by DON were observed,including reduced cellular DNA fragmentation,decreased release of lactate dehydrogenase(LDH),normalized ROS levels,restoration of extracellular Ca2+and K+contents to normal levels(P＜0.01),as well as suppressed the enzyme activities of caspase-1 and caspase-4(P＜0.01).Additionally,the mRNA expression levels ofTNF,MDP,NOD2,TLR4,ASC and GSDMD were significantly improved(P＜0.01),while both mRNA and protein expression levels of NF-κB,NLRP3,caspase-1,IL-1β and IL-18 were significantly upregulated(P＜0.01)in the CGA+DON group,compare to those in the DON group.Conclusion Pretreatment with 80 μmol/L CGA for 4 h effectively alleviated pyroptosis in IPEC-J2 cells induced by 2,500 ng/mL of DON through inhibiting activation of the NF-κB/NLRP3/capase-1 pathway.