摘要为了解先天性巨结肠(HD)病变肠段细胞能量代谢的状况.通过酶组织化学方法对20例HD患儿正常段、狭窄段全层组织,进行了五种酶的定性、定量及计数分析:①ATPase(腺苷三磷酸酶),②SDH(琥珀酸脱氢酶),⑧MAO(单胺氧化酶),④ChE(胆碱脂酶),⑤ChE+Ag(胆碱脂酶+镀银).发现狭窄段有如下变化:ATPase、SDH和ChE活性显著升高(P＜0.01);MAO活性显著降低(P＜0.01);粘膜下和肌间神经丛(ChE+Ag)减少1/3,神经节细胞数(SDH)减少4/5(P＜0.01).说明HD狭窄段细胞能量代谢是旺盛的,检测ATPase、DH、MAO可作为诊断HD新的参考.

abstractsFor further exploration of the cell energy metabolic changes in the spastic segment of Hirschsprung's disease (HD),five enzyme alterations namely:1.ATPase (adrenosinotriphosphatase),2.SDH(succinatodehydrogenase),3.MAO(monamine oxidase),4.ChE(cholinesterase).5.ChE+Ag(cholinesterase+Ag),in normal and spastic segment from 20 operated children of HD were studied by histochemical staining techniques,and examined and analyzed qualitatively and quantitatively.The results were as following:The activities of ATPase,numbers of the intermuscular plexus,submucosal plexus and ganglion cells reduced significantly in the spastic segment(P＜0.01).Conclusions:The cell energy metabolism of Hirschsprung's disease in the spastic segment is more active than that in normal segment.And the determination of the ATPase,SDH and MAO activities by enzyme-histochemical method may be suggested as a new method in the diagnosis of HD.