摘要目的 本实验以10ug/d上皮生长因子(EGF)皮下给药促进大白鼠小肠缺血再灌注损伤后粘膜的修复,观察细胞的增殖.方法 SD雄性大白鼠60只,阻断肠系膜上动脉后去除阻断为小肠缺血再灌注损伤模型,随机分为对照组(A)、皮下放置不含EGF缓释片组(B)和放置EGF缓释片组(C).观察各组0时(缺血60分钟再灌注120分钟后)、第2、4、8天组织学改变,并进行粘膜细胞DNA流式细胞仪分析.结果 组织学检查示B组再生绒毛较C组短小、稀少.肠粘膜细胞经流式细胞仪DNA分析,结果 表明48小时修复,C组S-期细胞(13.02%±1.39%)较B组升高更为明显(Cvs B P＜0.05),损伤修复后第4、8天,B组细胞增殖已趋于正常,与A组比较P＞0.05,而C组上皮细胞增殖仍居高不下,较B组和A组均有统计学意义(P＜0.01).结论 EGF不仅可促进缺血再灌注后小肠牯膜的再生修复,同时有助于消化吸收功能的早期恢复.

abstractsObjective To evaluate the influence of long-term continuous administration of epidermal growth factor(EOF)on mucosal cell proliferation after intestinal ischemia-reperfusion(I/R)injury.Methods Sixty male SD rats weighting 200-250 grams were divided into equal three groups:Group A(controls)received laparotory only:Group B underwent 60 minutes of SMA occlusion with EGF placebo pellets implanted subcutaneously and Group C SMA occlusion and administration of EGF.The bowels were examined for histology and isolating mucosal cells for DNA analysis by flow cytometry.Results Intestinal segments showed epithelial denudation and villi impairment after 60 minutes of ischemia and 2 hours with reperfusion in both I/R groups.The mucosa of all specimens healed with reepithelialized villus tips,but the villi were scarce and shorter in group B.In the DNA profile the percent of S-phase cells in group B and C were higher than that of the control(P＜0.01).At 2 days regeneration,it kept a higher level in group B (B vs A P＜0.01).The S-phase cells of group C(13.02% ± 1.39%)was the highest in the meantime(C vs B P＜0.05).At the 4-8 days period,it still kept a significantly higher level than that of group B.Conclusions Subcutaneous administration of EGF(10 ug/d)can stimulate crypt cell proliferation,mucosal regeneration and intestinal function recuperation.