摘要Metagenomic binning is the process of sorting genome or contig sequences obtained by metagenomic sequencing of environmental DNA or genetically heterogeneous groups of organisms into cate-gories representing the individual species or genetically homogenous groups(e.g.,strains)from which they originated(Rasheed and Rangwala,2013).Conventionally,whole-genome sequencing of sin-gle species requires culture and isolation of microorganisms.Howev-er,the vast majority of microorganisms in the environment are unculturable by current methods.Metagenomic binning methods thus help researchers to obtain the whole genome sequences of these non-culturable microorganisms and to predict the gene func-tions of previously uncharacterized species(Hazards et al.,2019).