Election Spin Resonance Studies of Free Radical Formation and Oxygen Consumption of Lens Epithelium During Ultraviolet Exposure
摘要A long life election spin resonance (ESR) signal at g=2.0006 was observed in the normal lens epithelium and cortical fibers.During ultraviolet(UV) exposure,a new ESR signal at g=2.0060 was found in the lens epithelium.But this specific signal was not detected in the lens cortical fibers.This suggested that lens epithelial cells were more susceptible to the free radi-cal formation which was induced by UV light.By means of ESR spin probe oxymetry,the oxygen uptake of lens epithelial cells was measured.The more the oxygen uptake,the higher the K value was.The K value of the oxygen con-sumption of epithelial cell linearly correlated with time of consumption(20-60 min) and increased as a function of UV exposure time(1-5 min).The oxygen consumption rate of lens epithelial cell was approximately 1.38 × 10s and in-creased to 7.1 × 10s O2 molecules per cell per sec.The oxygen consumption rate increased more than 5 times.These results indicates that UV light can ac-celerate the respiratory function of lens epithelial cells.The necessity of excess oxygen of lens epithelial cells may play a role in the cataract formation induced by UV light.Eye Science 1993;9:15-18.
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