摘要Purpose:To study the ability of Homoharringtonine (Hh),5-Fluorouracil (5-Fu),and Adriamycin (ADM) on inhibiting the proliferation of rabbit lens epithelium.Methods:Whole rabbit lenses were removed from freshly enucleated eyes under sterile condition.The rabbit lens eptithlia (RLE) were isolated and culatured:(1)The passage RLE were placed in 24-well tissue culture plates and incubated for 48 hours,then exposed to different concentrations of Hh,5-Fu,and ADM for 24 and 72hours;(2)The passage RLE and Hh(0.084μg/ml),5-Fu(0.058μg/ml),ADM (0.45ng/ml)were placed and cultured for 24 hours;(3)The morphological changes of RLE exposed to different concentrations of Hh,5-Fu and ADM were studied under light microscope.Results:The ID50 of Hh,5-Fu and ADM exposed to RLE for 24 hours were 0.84μg/ml,0.58μg/ml and 4.50ng/ml,respectively,and those for 72 hours were 0.49μg/ml,0.33μg/ml and 3.85ng/ml.The attachment rate of RLE after being cu-latured for 24 hours with Hh,5-Fu and ADM were respectinely 83.6％,89.1％and 87.3％.The morphological changes of RLE demonstrated that obvious changes in the cell membrane and cytoplasm were found even in lower concentra-tion,but changes in the nuclei could only be found in higher concentration of these drugs.Conclusion:Hh can not only inhibit the proliferation of RLE but also reduce the number of attached cells.It is suggested that Hh may be more useful for the pre-vention of after cataract than 5-Fu and ADM.