摘要Purpose:To analyze the change of water-soluble-protein (WSP),urea-soluble-protein (USP) and membrane intrinsic protein (MIP) in human senile catarct.Methods:The water-soluble-fractions (WSF) were prepared basically according to the method of Kibbelear,et al.But in this study,5mmol/L B-mereaptoethanol was added to the buffer solution.The urea-soluble-fractions (USF) were pre-pared basically according to the method of Kibbelear,et al.Lens fiber cell mem-branes were purified basically according to the method of Russell,et al.SDS-PAGE were performed according to the procedure of Laemmili,et al.using re-solving gel 13％ and 3％ stacking gel.Results:The WSP was fractionated into HM+α-,β1-3-and γ-crystallin compo-nents.In nuclear cataractous lenses HM+α-and B-erystallin increase,while r-crystallin decrease.The USP from clear lenses contains mainly αβ chains of 22KD,whereas in cataractous lenses,especially in nuclear cataractous lenses,the relative amount of the 28-and 23KD polypeptide (the components of β-crys-tallin) increased markedly.Lens fiber cell MIP,clear lens and cataract lens con-tained the main polypeptide of 27KD(MIP) and 23KD (MP23).Conclusion:The water-insolube protein,whether in quantity or in quality,plays an important role in cataract formation.