摘要目的探讨吲哚美辛(IN)对体外培养的人胚胎视网膜色素上皮(RPE)细胞的抑制作用及DNA合成的影响.方法分别用核酸蛋白分析仪测定加入50、100、200、400、600 μmol/L IN作用2h后RPE细胞DNA质量浓度的变化和MTT法测定加入100、200、400、600、800、1 000 μmol/L IN 12 h后RPE细胞数量的改变.结果前者其细胞DNA质量浓度(单位:μg/ml)分别为 101. 171 2± 15.512 4、 88.640 0 ±13. 584 5、72.365 1±7.796 9、5.908 9±10.722 9、51.223 6±8.775 7.与对照组213.735 1±83.157 2相比,差异有显著性(q值分别为5.481、6.091、6.883、7.490、7.912,P值分别为0.000、0.000、0.000、0.000、0.000);后者其细胞A值分别为0.236 7±0.054 6、0.168 7±0.069 5、0.081 9±0.034 6、0.065 6±0.017 6、0.055 4±0.028 6、0.050 8±0.027 7.与对照组0.267 4±0.043 0相比,差异有显著性(q值分别为1.442、4.621、8.682、9.447、9.925、10.140,P值分别为0.158、0.000、0.000、0.000、0.000、0.000).结论IN可抑制体外培养的RPE细胞的DNA合成及增殖.

abstractsObjectiveTo investigate the inhibitory effects of indomethacin(IN) on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium(hRPE) cells in vitro.MethodsPrimary culture and subculture of hRPE cells were established in vitro first.Cultured hRPE cells were treated by various concentrations 50,100,200,400,600 μ mol/L(final concentration)of IN for 24h.After 24h,the amount of DNA in RPE cells was determined by the absorbance at 280nm of Nucleic Acid δ Protein Analysis.Cells proliferation of RPE were measured with methyl thiazolyl tetrazolium(MTT) assay method by adding 100,200,400,600,800,1000μ mol/L(final concentration) of IN for 12h.ResultsAfter added various concentrations of IN,the DNA concentrations were ( 101.1712± 15.5124),( 88.6400± 13.5845),( 72.3651± 7.7969),( 59.9089± 10.7229),( 51.2236± 8.7757)μg/ml respectively,P values were 0.000,0.000,0.000,0.000,0.000(q test) as compared to that ( 213.7351± 83.1572)μg/ml in 0μg/L IN.The A values added 100,200,400,600,800,1000μmol/L of IN were ( 0.2367± 0.0546),( 0.1687± 0.0695),( 0.0819± 0.03461),( 0.0656± 0.01759),( 0.0554± 0.02865),( 0.0508± 0.02775)respectively,P values were 0 .158,0.000,0.000,0.000,0.000,0.000(q test) as compared to ( 0.2674± 0. 04302) of A value of 0ug/L IN.ConclusionThe data suggested that IN can inhibit DNA synthesis and proliferation of hRPE cells in vitro in a dose dependent manner.