摘要背景 增生性玻璃体视网膜疾病包括所有眼内过度增生性病变,许多细胞因子在其发病中起重要作用.缺氧诱导因子-1α(HIF-1α)参与多种缺血性疾病的发生发展,但是否在增生性玻璃体视网膜病变(PVR)发病中起作用尚不清楚.目的 检测增生性玻璃体视网膜疾病患者玻璃体中HIF-1α的质量浓度,探讨HIF-1α在增生性玻璃体视网膜疾病发病中的作用.方法 在常规玻璃体切割术中收集玻璃体标本.实验组共69例71眼,其中包括增生型糖尿病视网膜病变(PDR)患者37例39眼,PVR患者32例32眼;病例对照组16例16眼,其中包括黄斑裂孔(MH)14例14眼,黄斑前膜(ERM)2例2眼;正常对照组8例8眼.采用酶联免疫吸附测定法(ELISA)检测受检眼玻璃体中HIF-1α的质量浓度.结果 实验组、病例对照组及正常对照组玻璃体中HIF-1α质量浓度分别为(294.08±2.97)、(260.41±8.29)、(16.38±3.56)mg/L,3组玻璃体中HIF-1α质量浓度比较差异有统计学意义(F=248.77,P=0.00),其中实验组玻璃体中HIF-1α质量浓度高于正常对照组及病例对照组,差异有统计学意义(t=22.25,P=0.00;t=2.70,P=0.00),病例对照组玻璃体中HIF-1α质量浓度高于正常对照组,差异有统计学意义(t=14.21,P=0.00),PVR患者玻璃体中HIF-1α质量浓度与眼病史之间关联性较弱.结论 增生性玻璃体视网膜疾病患者玻璃体中HIF-1α质量浓度增高,但与眼病史关联性较弱,HIF-1α可能在增生性玻璃体视网膜疾病发病中起一定作用.

abstractsBackground Proliferative vitreo-retinal diseases include all the overly proliferative diseases of eyes.Many kinds of cytokines play important roles in their pathogenesis.Hypoxia-inducible factor 1α(HIF-1α)participates in the pathogenesis of a lot of ischemic diseases.HIF-1α may play an important role in the pathogenesis of proliferative vitreo-retinal diseases. Objective Present study was to observe the expression of HIF-1α in vitreous body in the eyes with proliferative vitreo-retinal diseases.The vitreous samples were collected during the vitrectomy from 71 eyes of 69 patients(experimental group)including 39 eyes with proliferative diabetic retinopathy(PDR)and 32 eyes with proliferative Vitreoretinopathy(PVR).Sixteen eyes of 16 patients as the cases control group,including 14 eyes with macular hole(MH)and 2 eyes with epiretinal macular membrane(ERM).What is more,there were 8 vitreous fluid samples of the normal control group.The concentrations of HIF-1α in vitreous body were detected by enzyme-linked immunosorbent assay(ELISA).The written informed consent was obtained from each patient before any medical procedure. Results The concentrations of HIF-1α in vitreous body in the eyes of experimental group,the cases control group and the normal control group were(294.08±2.97)、(260.41±8.29)、(16.38±3.56)mg/L respectively,showing a significant difference among the three groups(F=248.77,P=0.00).The concentrations of HIF-1α in vitreous body were obviously increased in the eyes of experimental group in comparison with the normal control group and the cases control group respectively(t=22.25,P=0.00;t=2.70,P=0.00 respectively).The concentrations of HIF-1α in vitreous body were increased in the eyes of the cases control group in comparison with the normal control group eyes(t=14.21,P=0.00).There was inferior correlation between the concentrations of HIF-1α in vitreous body in the eyes of experimental group and the course of ophthalmopathy. Conclusion Intravitreous HIF-1α level regulates in the eyes with proliferative vitreo-retinal diseases. Expression of HIF-1α in vitreous had inferior correlation with the course of ophthalmopathy. HIF-1α may play an important role in the pathogenesis of proliferative vitreo-retinal diseases.