摘要背景 准确的眼压测量是青光眼实验研究的基础.TonoLab是一种新型的动物眼压计,其测量眼压的可重复性尚有待验证. 目的 评估新型眼压计TonoLab在遗传性高眼压模型中的准确性. 方法 采用新型眼压计TonoLab对麻醉状态下的63只7月龄遗传性高眼压DBA/2J小鼠进行眼压测量,测量时由两位技术娴熟的操作者分别、连续测量同一只眼的眼压3次,测量不分先后顺序,采用操作者盲法获取测量数据.计算测量结果的组内标准差(Sw)、组内相关系数(ICC)及组内变异系数(CVw),以评估每位操作者测量结果的可重复性,采用Bland-Altman一致性检验评估两位操作者之间测量结果的可重复性. 结果 共测量63只小鼠126只眼,其中正常眼压者105只眼,操作者1测得的平均眼压为(12.98±1.05) mmHg(1 mmHg=0.133 kPa),操作者2为(12.41±0.92) mmHg;高眼压者21只眼,操作者1和操作者2测得的平均眼压分别为(27.76±1.95) mmHg和(27.49±1.28) mmHg.正常眼压组两位操作者测量结果的Sw、ICC及CVw分别为1.34/1.11 mmHg、0.90/0.92和8.24％/7.42％;高眼压组两位操作者测量结果的Sw、ICC及CVw分别为2.21/1.49 mmHg、0.89/0.95和6.97％/4.82％.正常眼压组两位操作者测量结果之间的平均偏差为0.60 mmHg[95％可信区间(CI):-5.20 ～6.30 mmHg],高眼压组为0.30 mmHg(95％ CI:-7.30～ 7.80 mmHg).结论 TonoLab眼压计在正常眼压及高眼压小鼠中单个操作者测量结果的组内可重复性高,但两位操作者测量结果之间的可重复性较低,因此单个操作者用TonoLab眼压计测得的眼压结果可靠、准确.

abstractsBackground Reliable intraocular pressure (IOP) measurement is essential in glaucomatous study.TonoLab is a newly designed tonometer for small animals,however,its repeatability and reproducibility remains unknown.Objective This study aimed to evaluate the repeatability and reproducibility of TonoLab rebound tonometer in DBA/2J mice.Methods Intraocular pressure was measured in both eyes of 63 DBA/2J mice aged 7 months under systemic anesthesia with TonoLab rebound tonometer.IOP of each eye was measured for 3 times by two observers independently and consecutively using TonoLab rebound tonometer,and the data were obtained in single blind method.Within standard deviation (Sw),interclass correlation coefficient (ICC),within coefficient of variation (CVw) and corresponding 95％ confidence interval (CI) were calculated to evaluate the repeatability of measuring result from single observer,and Bland-Altman method was used to assess the agreement between two observers.The abnormal data which affected repeatability was analyzed.Results Among the 126 eyes,105 eyes showed the normal IOP with mean value (12.98 ± 1.05) mmHg from observer 1 and (12.41 ±0.92) mmHg from observer 2.Ocular hypertension was found in 21 eyes with mean value (27.76 ± 1.95) mmHg and (27.49 ± 1.28) mmHg for observer 1 and observer 2 respectively.Sw,ICC and CVw for observer 1/observer 2 were 1.34/1.11 mmHg,0.90/0.92,8.24％/7.42％ in normal eyes and 2.21/1.49 mmHg,0.89/0.95,6.97％/4.82％ in hypertensive eyes.The average bias between two measuring values from different observers was 0.60 mmHg in the normal eyes (95％ CI:-5.20-6.30 mmHg) and 0.30 mmHg in the hypertensive eyes (95％ CI:-7.30-7.80 mmHg).Conclusions TonoLab rebound tonometer can offer a good intraobserver repeatability in both normal and hypertensive eyes in mice,but the agreement between two observers is outside an acceptable level.So we consider IOP in mice obtained by TonoLab rebound tonometer is reliable and accurate from a single observer.