摘要目的 探讨重组人血小板源性生长因子-BB(rhPDGF-BB)对人视网膜血管内皮细胞(hRVECs)增生和迁移的影响及其作用机制. 方法 采用含体积分数10％胎牛血清的DMEM培养液培养hRVECs,分别将10、50和200 ng/ml rhPDGF-BB加入对数生长期hRVECs的培养液,未添加rhPDGF-BB者作为正常对照组.采用细胞计数试剂盒-8(CCK8)法检测各组细胞的增生情况;采用细胞划痕法检测细胞相对迁移面积(迁移后无细胞区面积/划痕初期无细胞区面积);采用逆转录PCR法检测hRVECs中rhPDGF-BB受体(rhPDGF-BBR)mRNA的相对表达量;采用实时荧光定量PCR法检测hRVECs中VEGF mRNA和整合素mRNA相对表达量.结果 培养的hRVECs生长良好,用rhPDGF-BBR引物能扩增出与引物设计长度相符的表达条带.正常对照组及10、50和200 ng/ml rhPDGF-BB组培养细胞后24 h细胞增生值(A)分别为1.01 ±0.05、1.09±0.04、1.10±0.02和1.13±0.05,10、50和200 ng/ml rhPDGF-BB组A值明显高于正常对照组,差异均有统计学意义(t=2.504、3.430、3.483,均P＜0.05);细胞划痕试验后24 h,正常对照组及10、50和200 ng/ml rhPDGF-BB组细胞相对迁移面积分别为0.42±0.10、0.38±0.09、0.55±0.06和0.61 ±0.05,划痕试验后48 h细胞相对迁移面积分别为0.75±0.06、0.81±0.02、0.87±0.02和0.98±0.02,总体比较差异均有统计学意义(F分组=16.283,P=0.000;F时间=209.129,P=0.000),随着rhPDGF-BB剂量增加和作用时间延长,细胞相对迁移面积均明显增加;实时荧光定量PCR法检测显示正常对照组及10、50和200 ng/ml rhPDGF-BB组hRVECs中整合素mRNA相对表达量分别为1.06±0.02、1.30±0.10、1.20±0.16和1.27±0.08,VEGF mRNA相对表达量分别为0.97±0.05、1.06±0.16、1.58±0.18和1.66±0.21,其中50 ng/ml和200 ng/ml rhPDGF-BB组细胞中整合素mRNA及VEGF mRNA相对表达量均明显高于正常对照组,差异均有统计学意义(整合素mRNA:t=3.900、4.014,均P＜0.05;VEGF mRNA:t=6.940、7.210,均P＜0.05). 结论 rhPDGF-BBR促进hRVECs的增生和迁移,其作用呈剂量和时间依赖性,其可能与上调VEGF和整合素在细胞中的表达有关.

abstractsObjective This study was to investigate the role of recombinant human platelet derived growth factor-BB(rhPDGF-BB) in the proliferation and migration of human retinal vascular endothelial cells (hRVECs).Methods hRVECs were cultured in DMEM with 10％ fetal bovine serum.The rhPDGF-BB at the concentrations of 10,50 and 200 ng/ml were added into the medium of exponential phase-growth cells for 24 and 48 hours,respectively,and no rhPDGF-BB was added in the normal control group.The proliferation of the cells (absorbancy) was assayed by cell counting kit 8 (CCK8) method.Cell scratch test was employed to evaluate the relative migration area of cells (migrated acellular area/initial acellular area).The relative expression of rhPDGF-BB recepter (rhPDGF-BBR) mRNA in the cells was detected by reverse transcription PCR.The relative expression of VEGF mRNA and integrin mRNA in the cells was detected using real-time fluorescence quantitative PCR.Results hRVECs grew well and a expressing band according with rhPDGF-BBR prime was displayed.The absorbancy values of thecells were 1.01±0.05,1.09±0.04,1.10±0.02 and 1.13±0.05 in the normal control group and 10,50,200 ng/ml rhPDGF-BB groups at 24 hours after culture,and those in the 10,50 and 200 ng/ml rhPDGF-BB groups were significantly increased in comparison with the normal control group (t =2.504,3.430,3.483,all at P＜0.05).The relative migrated areas of the cells in the normal control group and 10,50,200 ng/ml rhPDGF-BB groups were 0.42±0.10,0.38±0.09,0.55±0.06 and 0.61±0.05 at 24 hours after culture,and those at 48 hours were 0.75±0.06,0.81 ±0.02,0.87±0.02 and 0.98±0.02,showing significant differences among the groups (Fgroup =16.283,P =0.000;Ftime =209.129,P=0.000),and the relative migrated areas was depended upon the rhPDGF-BB dose and time.The relative expressions of integrin mRNA were 1.06 ± 0.02,1.30 ±0.10,1.20 ± 0.16 and 1.27 ± 0.08,and those of VEGF mRNA were 0.97±0.05,1.06±0.16,1.58 ±0.18 and 1.66 ±0.21 in the normal control group and 10,50 ng/ml,200 ng/ml rhPDGF-BB groups,respectively,and increased expressions of integrin mRNA and VEGF mRNA were found in the 50 and 200 ng/ml rhPDGF-BB groups compared with the normal control group (integrin mRNA:t =3.900,4.014,both at P ＜ 0.05;VEGF mRNA:t =6.940,7.210,both at P ＜ 0.05).Conclusions rhPDGF-BB/rhPDGF-BBR signal promotes the proliferation and migration of hRVECs probably by up-regulating the expressions of integrin and VEGF.