摘要目的 探索离体视网膜神经节细胞(RGC)在不同色光刺激下电活动反应的作用规律.方法 选取3周龄SPF级C57BL/6小鼠30只,制作离体全视网膜铺片.通过膜片钳系统on cell模式记录RGC在蓝光(400 nm)、绿光(580 nm)和白光3种色光刺激下RGC的电活动参数;将所有RGC根据不同色光反应分为400 nm色光敏感RGC、580 nm色光敏感RGC和色光不敏感RGC,进一步根据光反应类型、响应模式将RGC分为ON型、ON/OFF型和OFF型.组间比较不同色觉类型RGC的基线放电模式(放电基线频率及簇发放电频率)和光反应放电模式(响应模式分布、光反应放电频率及放电频率增幅).结果 共记录82个RGC的色光反应信息.RGC的自发放电活动频率范围为0.00 ～ 32.33 Hz.400 nm色光敏感RGC为52个,占63.41％;580 nm色光敏感RGC共29个,占35.37％;色光不敏感RGC 1个,占1.22％.400 nm敏感组的OFF型RGC比例为32.69％,高于580 nm敏感组的0.00％;580 nm敏感组的ON/OFF型瞬时响应RGC比例为34.48％,高于400 nm敏感组ON/OFF型瞬时响应RGC的7.69％;400 nm敏感RGC与580 nm敏感RGC组间光刺激响应模式分布比较,差异有统计学意义(x2=18.069,P=0.000).580 nm敏感RGC放电频率增幅为(22.93±10.23)Hz,显著高于400 nm敏感RGC的(14.44±10.11)Hz,580 nm敏感ON型RGC放电频率增幅为(24.17±8.98)Hz,显著高于400 nm敏感ON型RGC的(11.12±10.35)Hz,差异均有统计学意义(t=4.060,P=0.044;t=5.373,P=0.021),其余放电信息的组间比较差异均无统计学意义(均P＞0.05). 结论 从RGC色觉敏感角度对RGC分类所得动作电位反应规律无明确特异性,需进一步实验以验证能否通过电刺激参数模拟某类色觉RGC激活后的放电模式,从而产生色觉.

abstractsObjective To explore the reaction pattern rules of mouse retinal ganglion cells potential under different wavelengths of light stimulation.Methods Thirty SPF grade 3-week-old C57BL/6 mice were used for ex vivo whole mount retina preparation.The cells firing activities were recorded on patch clamp system with on cell touch mode under stimulation of 400 nm,580 nm and white light,respectively.According to different reactions to different light stimulation,the cells were classified into 400 nm sensitive RGC,580 nm sensitive RGC and color vision insensitive RGC.Then the cells were further classified according to light ON type,light ON/OFF type or light OFF type.The RGC's baseline firing pattern (baseline firing frequency,burst firing frequency) and light activation firing pattern (response pattern,light response firing frequency,light response firing amplification) were compared among different RGC classifications.Results Eighty-two RGCs were recorded in total.The frequency of spontaneous firing activity ranged from 0.00 Hz to 32.33 Hz among different RGCs.400 nm sensitive RGCs were 52(63.41％),580 nm sensitive RGCs were 29(35.37％) and color vision insensitive RGC was 1 (1.22％).OFF type RGC was the main cell type in 400 nm sensitive group (36.29％),and ON/OFF type RGC was the main cell type in 580 nm sensitive group (34.48％).The firing amplification in 580 nm sensitive RGC was (22.93± 10.23)Hz,which was significantly higher than (14.44± 10.11) Hz in 400 nm sensitive RGC (t =4.060,P =0.044).The firing amplification in 580 nm sensitive ON type RGC was (24.17±8.98)Hz,which was significantly higher than (11.12± 10.35)Hz in 400 nm sensitive ON type RGC (t =5.373,P =0.021).Conclusions There is no specific firing pattern rules among different light sensitive RGCs.In the future,artificial color vision may be achieved through personalized electric stimulation and learning feedback strategy.