摘要The sugarcane subtribe Saccharinae(Andropogoneae,Poaceae)was established in 1846,but its delimi-tation has long been debated.Moreover,the relationships among the genera of Saccharinae remain unclear,and there is no consensus on whether Pseudosorghum,a small genus in tropical Asia with only two species,should be included.Here,we performed phylogenomic analyses using whole plastomes(69 of them newly sequenced)from 132 individuals,representing 65 species in 19 related genera.We also built trees with nuclear ribosomal DNA sequences.Our results justify the inclusion of Pseudosorghum,likely also the Eulalia Clade Ⅲ,in Saccharinae.Furthermore,both morphological and molecular analyses support merging the two Pseudosorghum species.The backbone relationships of the Saccharinae phy-logeny were highly supported with four polyphyletic clades of Miscanthus and the inclusion of Narenga and Tripidium rufipilum in Saccharum.Pseudosorghum is moderately supported as sister to the Miscanthus Clade I,while the remaining Tripidium species could be excluded from the subtribe.Saccharinae is estimated to have originated～3.73 million years ago in East Asia,followed by intercontinental dispersals.Our study provides a comprehensive phylogenetic framework for future taxonomic revisions of this economically important subtribe.