摘要Objective: To compare the antibacterial property between tissue cultured and wild plants ofClitoria ternatea. Methods: Callus formation, shoot multiplication and rooting were carried out in MS medium supplemented with 2,4-D and KN; BA, KN and IAA; NAA and NAA, respectively. The antibacterial activity was evaluated using disc diffusion method against the gram positive bacteria Bacillus cereus, Bacillus subtilis and Staphylococcus aureus, the gram negative Klebsiellapneumonia, Proteus vugaris and Salmonella typhi. Soxhlet apparatus was used for extraction with a series of solvents methonolic, perolium ether and ethyl acetate. Results: The best shoot initiation was resulted in KN (1.0 mg/L) and IAA (0.5 mg/L).The maximum shoot multiplication was obtained by BA (0.75 mg/L) and IAA (0.5 mg/L). The maximum callus initiation was observed by adding 2,4-D (1.0 mg/L) and KN (0.5 mg/L) using leaf explants. Callus differentiation was achieved by addition of 2, 4-D (0.3 mg/L) and KN (0.3 mg/L). The combination of plant growth regulators such as 2,4-D and KN induced best callus in all the explants of Clitoria ternatea compared to other hormonal combination. Maximum rooting was achieved by adding NAA (1.0 mg/L) on half strength MS medium. The methonolic extract of callus showed higher activity in Klebsiella pneumonia and Staphylococcus aureus than rest of the organisms. Conclusions: From the present investigation, it can be concluded that in vitro derived callus and plants of Clitoria ternatea exhibits antibacterial activity against certain pathogenic bacteria.