首页 > 亚太热带医药杂志（英文版） > Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool：Validation method using strains from Colombia classified according to their discrete typing unit

Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool：Validation method using strains from Colombia classified according to their discrete typing unit

导出在线阅读解读下载全文终端阅读

二维码有效期 120s

点击刷新

注：终端设备浏览有效期：

学术成果认领

打印收藏纠错

摘要Objective:To classify 21 new isolates of Trypanosoma cruzi (T. cruzi) according to the Discrete Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to detect the parasite in biological samples. Methods: Strains were isolated, DNA extracted, and classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used along with other isolates of various biological samples, for a new PCR using primers designed. Finally, the amplified fragments were sequenced. Results: It was observed the predominance of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no band was obtained when other species were used. Conclusions:This work reveals the genetic variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for detecting the presence of T. cruzi.

作者单位 Grupo de Parasitología Molecular, Departmento de Parasitología Universidad de Granada, Severo 0choa s/n, E-18071 Granada Espa?a ^[1] Laboratorio de Zoonosis y ETV′s, C.I.R. Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Ave. Itzaes #490 x 59, 97000 Mérida, Yucatan Mexico ^[2] Grupo Biología y Control de Enfermedades Infecciosas BCEI-SIU, Instituto de Biología, Universidad de Antioquia, Medellín, Colombia ^[3]

关键词 Trypanosoma cruzi Polymerase chain reaction Colombia Superoxide dismutase gene b-based primers Discrete typing unit

DOI 10.1016/S1995-7645(14)60149-8

发布时间 2015-11-05（万方平台首次上网日期，不代表论文的发表时间）

基金项目

This project is funded by a FPU a grant from the Ministry of Education of Spain（Foundotion project）