Effects of regenerated tissue extracts after liver injury on the proliferation, differentiation, migration and invasion of SK-HEP1 cells

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摘要Objective:To study the effects of regenerated tissue extracts after liver injury on the proliferation,differentiation,migration and invasion of SK-HEP 1 cells.Methods:Regenerated tissue extracts after liver injury were used to induce SK-HEP1 cells after enrichment,their effects on the proliferation,differentiation,migration and invasion of SK-HEP1 cells were observed through in vitro cell culture,MTT,flow cytometry and transwell assays.Results:In response to the action of regenerated tissue extracts after liver injury,SK-HEP1 cells were blocked in G0/G1 phase,their growth rate was distinctly reduced.The number of SK-HEP1-fj colonies decreased.The migration ability of SK-HEP1 cells showed a decreased trend on day 7 and day 11 after induction.SK-HEP1's invasion ability clearly decreased on days 7 and 11 after induction,especially on day 7.Conclusions:To a certain extent,regenerated tissue extracts after liver injury can inhibit the proliferation,differentiation,migration and invasion of hepatoma cells,showing an important potential of being a differentiating agent for the treatment of liver cancer.

作者单位 Department of Pathology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China ^[1] Emergency Department, The First Affiliated Hospital of Hainan Medical University, Haikou, Hainan Province, China ^[2] Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China ^[3] Department of Oncology, Nanfang Hospital, Southern Medical University , Guangzhou 510515, Guangdong Province, China ^[4] Department of Pathology , Sun Yat-sen University Cancer Center, Guangzhou 510060, Guangdong Province, China ^[5] Department of Pathology, Nanhai District People's Hospital, Foshan 528200, Guangdong Province, China ^[6] Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China;Pathology Teaching and Research Section, Sun Yat-sen University Zhongshan School of Medicine, Guangzhou 510080, Guangdong Province, China ^[7]

关键词 SK-HEP1 hepatoma cell Regenerated tissue extracts of liver injury Differentiation-inducing therapy SK-HEP1 cell enrichment (SK-HEP1-fj)

栏目名称

Original articles

DOI 10.4103/1995-7645.228439

发布时间 2018-06-19

基金项目

We thank Prof Min Li for the preparation of the manuscript and cover letter and thank all the staff of Pathology Division of the First Affiliated Hospital of Sun Yat-sen for their strong support of this experiment

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