摘要Objective:To evaluate the phenotypic resistance profile of Aedes(Ae.)albopictus against multiple insecticides and to delineate the frequency and pyrethroid-resistance association of knockdown resistance(kdr)mutations V1016G,I1532T and F1534S in Ae.albopictus in Pudong New Area,Shanghai,China.Methods:From June to July 2024,a total of 1 249 Ae.albopictus eggs and 4 732 adults were collected from five towns in the Pudong New Area.These collected specimens were then reared to obtain the F1 generation including 10 235 larvae at the late 3rd to early 4th instar stages,as well as 5 800 non-blood-fed adults aged 3 to 5 days.Larval resistance was assessed via the immersion method,and adult resistance via the contact tube method.For the larval,0≤resistance ratio(RR)<3 indicates sensitivity;3≤RR<10 denotes low resistance;10≤RR<40 signifies moderate resistance;RR≥40 represents high resistance.While for adults,a mortality rate of≥98%stands for sensitivity,80%≤mortality rate<98%indicates suspected resistance,and mortality rate<80%denotes a resistant population.A pooled sequencing strategy(8 sample pools)was used to detect genotype kdr mutations including V1016G,I1532T,F1534S in the voltage-gated sodium channel(VGSC)gene.Results:Ae.albopictus larvae displayed low resistance to fenthion(RR=5.85),temephos(RR=4.63)and β-cypermethrin(RR=3.20),but remained fully susceptible to propoxur(RR=0.33).Adults showed incipient resistance to β-cypermethrin(mortality:92.7%),lambda-cyhalothrin(mortality:96.8%)and permethrin(mortality:86.6%),while remained sensitive to propoxur(mortality:100%).For Ae.albopictus population in the Pudong New Area,pyrethroid tolerance was primarily driven by F1534S mutation,which was amplified when the mutation was paired with V1016G or I1532T.Conclusions:These findings highlight the need for integrated resistance monitoring and targeted insecticide rotation—such as prioritizing propoxur for Ae.albopictus larval and adult control—to mitigate resistance spread and preserve vector management efficacy.