摘要Objective:To analyse the prevalence of serotypes,antibiotic resistance,and virulence genes of Group B Streptococcus(GBS)strains isolated from pregnant women at 35-37 weeks of gestation in Ho Chi Minh City,Vietnam,from January 2022 to January 2023.Methods:GBS strains were isolated through selective culture methods and confirmed by PCR.Serotyping,virulence gene detection,and antibiotic susceptibility testing were performed using PCR,gel electrophoresis techniques and Kirby-Bauer test.Results:Totally,61 GBS isolated from 300 participants have been identified including seven GBS serotypes(Ⅰa,Ⅰb,Ⅱ,Ⅲ,Ⅳ,Ⅴ,and Ⅵ).Serotypes Ⅶ,Ⅷ,and Ⅸ were not detected in the study population.Antibiotic resistance patterns varied:13.1%of isolates were fully susceptible,while the majority showed multi-drug resistance,with 34.4%resistant to three antibiotics.Serotype Ⅰa demonstrated high susceptibility(35.7%),while serotype Ⅲ showed extensive resistance,with 87.5%being resistant to at least three antibiotics.All strains are susceptible to vancomycin and β-lactams susceptibility also remained high,but resistance to clindamycin,erythromycin,and tetracycline was high(＞65%).The virulence genes scpB,cylB,fbsB,and cfb were highly prevalent(90%-100%),indicating their potential for vaccine and diagnostic development.Conclusions:Our findings provide valuable insights into GBS serotypes,resistance,and virulence factors,contributing to community monitoring,preventive measures,diagnostics,and vaccine development.However,the limited sample size necessitates further research.