摘要目的 探讨不同浓度葡萄糖对POAG患者小梁网细胞凋亡的影响,研究葡萄糖与POAG以及糖尿病与POAG之间的关系,进一步探讨POAG的发病机制.方法 实验研究.采用组织块培养法原代培养POAG患者小梁网细胞,取传代的小梁网细胞分别加入含葡萄糖终浓度为0.0(对照组)、5.5、45.0 mmol/L的无血清培养基,分别培养48 h后,采用CCK-8和流式细胞仪法检测不同浓度葡萄糖对小梁网细胞的影响.数据采用单因素方差进行分析.结果 通过CCK-8检测发现,随着浓度的增加,葡萄糖对小梁网细胞的增殖抑制作用增强,可促进细胞凋亡,且各实验组与对照组之间相比差异有统计学意义(F=13.87,P＜0.01);通过流式细胞仪法检测葡萄糖浓度为5.5、45.0 mmol/L实验组小梁网细胞凋亡率分别为10.65％±0.03％、25.74％±0.02％,均高于对照组(4.02％±0.03％),且差异有统计学意义(F=25.34,P＜0.01).结论 高糖可以使小梁网细胞的增殖能力下降,凋亡率增加,从而可能导致小梁网细胞网状结构改变,房水流出途径的阻力增加.

abstractsObjective To investigate the effect of different concentrations of glucose on trabecular meshwork cells in patients with primary open-angle glaucoma (POAG);to study glucose and POAG as well as the relationship between diabetes and POAG;and to further understand the pathogenesis of POAG.Methods In this experimental study,human trabecular meshwork cells were first cultured and then subcultured.The cells were incubated with different doses of glucose (0,5.5,45.0 mmol/l)and the final concentration was diluted with DMEM/F12 without serum for 48 hours.CCK-8 and flow cytometry were used to study the effect of glucose on the apoptosis of the cultured trabecular meshwork cells.Data were analyzed using ANOVA.Results With an increase in glucose concentration,CCK-8detected the inhibition of trabecular meshwork cells.The difference between the experimental group and control group was statistically significant (F=13.87,P＜0.01).Flow cytometry showed that the apoptosis rates in the experimental group with the different doses of glucose (5.5,45.0 mmol/L) in the final concentrations were 10.65％±0.03％ and 25.74％±0.02％,which were higher than the control group (4.02％±0.03％).The difference between the experimental group and control group was statistically significant (F=25.34,P＜0.05).Conclusion High glucose levels can cause a decrease in the proliferation of trabecular meshwork cells and increase the rate of apoptosis.This changes the network structure of trabecular meshwork cells,and maybe changes the resistance in the aqueous outflow pathway.