摘要目的 研究极低频电磁场(ELF-EMF)作用下人胚胎眼巩膜成纤维细胞(HFSF)中基质金属蛋白酶-2(MMP-2)活性与蛋白表达的变化以及ELF-EMF对Ⅰ型胶原(Collagen Ⅰ)合成的影响.方法 实验研究.根据是否暴露于50 Hz、0.2 mT电磁场或加入MMP-2特异性抑制剂(sc-204092),将实验细胞分为对照组、辐照组和抑制剂组.明胶酶谱法检测各组HFSF中MMP-2酶活性的变化;Western Blot法检测各组HFSF中MMP-2、Collagen Ⅰ蛋白水平的变化.采用单因素方差分析进行数据分析.结果 3组HFSF细胞中MMP-2酶活性、MMP-2、Collagen Ⅰ蛋白表达比较差异均具有统计学意义(F=14.96、139.88、63.46,P均＜0.01);辐照组较对照组MMP-2酶活性和蛋白表达升高(P＜0.05),Collagen Ⅰ蛋白表达下降(P＜0.01);抑制剂组较辐照组MMP-2酶活性和蛋白表达下降(P＜0.01),Collagen Ⅰ蛋白表达升高(P＜0.05).结论 ELF-EMF可能通过影响体外培养的HFSF中MMP-2的酶活性和蛋白表达来调节Collagen Ⅰ的合成.

abstractsObjective To observe the matrix metalloproteinase 2 (MMP-2) activity and protein expression in human fetal scleral fibroblasts (HFSFs) exposed to an extremely low frequency electromagnetic field (ELF-EMF) and its impact on collagen Ⅰ synthesis.Methods This was an experimental study.HFSFs were cultured and divided into a control group,radiation group and inhibitor group according to their exposure to 50 Hz,0.2 mT electromagnetic field or joint MMP-2 specific inhibitors (sc-204092).MMP-2 activity was measured by gelatin enzymography.MMP-2 and collagen Ⅰ protein expression were detected by Western Blot.A single factor analysis of variance was used to determine whether there was a difference among these groups.Results There were significant differences between the three groups (F=14.96,139.88,63.46,P＜0.01).Compared to the control group,MMP-2 activity and protein expression increased in the radiation group (P＜0.05) and collagen Ⅰ protein expression decreased (P＜0.01).Compared to the radiation group,MMP-2 activity and protein expression decreased in the inhibitor group (P＜0.01) and collagen Ⅰ protein expression increased (P＜0.05).Conclusion ELF-EMF may regulate the synthesis of collagen Ⅰ by affecting the activity and protein expression of MMP-2.