摘要目的 探讨肝细胞生长因子激活物抑制因子-1(HAI-1)基因在肾癌组织中的表达及其与肿瘤血管生成的关系.方法 应用半定量反转录-聚合酶链反应(RT-PCR)法检测HAI-1基因在56例肾癌组织和相应癌旁非癌肾组织及12例正常肾组织中的表达水平；采用免疫组织化学SP法测定上述组织中CD34的表达,并测定微血管密度(MVD),分析HAI-1基因的表达与肾癌临床病理特征及MVD的关系.结果 HAI-1基因在肾癌组织、癌旁非癌肾组织、正常肾组织中的阳性率分别为58.93％ (33/56)、76.79％ (43/56)和91.67％(11/12),肾癌组织中HAI-1基因阳性率显著低于癌旁非癌肾组织和正常肾组织(P＜ 0.05)；HAI-1基因的表达与Fuhrman病理分级、Robson临床分期呈负相关(r＝ -0.617、-0.432,P＜0.01或＜0.05)；肾癌组织有淋巴结转移者的HAI-1基因阳性率[35.29％(6/17)]显著低于无淋巴结转移者[69.23％( 27/39)],差异有统计学意义(P＜0.05).肾癌组织中MVD显著高于癌旁非癌肾组织和正常肾组织,差异有统计学意义(P＜0.05)；肾癌组织中HAI-1基因的表达与MVD呈负相关(r＝-0.516,P＜0.01).结论 HAI-1基因表达缺失与肾癌的发生和发展、浸润和转移存在一定的关系,HAI-1基因表达缺失可作为肾癌浸润、转移和预后的一种重要分子标志.

abstractsObjective To investigate the expression of hepatocyte growth factor activator inhibitor- 1 (HAl-1) gene in the tissues of renal cell carcinoma (RCC) and the correlation with tumor angiogenesis.Methods The expression of HAI-1 gene was detected by semi-quantitative RT-PCR in the tissues of 56 cases of RCC,corresponding adjacent non-cancer tissues and 12 nomad renal tissues,the expression of CD34was detected by immunohistochemistry SP method in the above-mentioned organizations,and counted the microvessel density (MVD),analyzed the relationship between the expression of HAI-1 gene in RCC and clinicopathological parameters,MVD.Results The positive expression rate of HAI-1 gene in RCC tissues,the adjacent non-cancer tissues and normal renal tissues were 58.93％(33/56),76.79％(43/56) and 91.67％(11/12),respectively.The expression of HAI-1 gene was a negative correlation to the Fuhrman histological grade,Robson clinical stage (r ＝ -0.617,-0.432,P ＜ 0.01 or ＜ 0.05 ).The positive expression rate in lymph node metastasis was significantly lower than that in without lymph node metastasis [ 35.29％(6/17) vs.69.23％(27/39),P＜ 0.05 ].The MVD in RCC tissues was significantly higher than that in the adjacent noncancer tissues and normal renal tissues(P＜ 0.05).The expression of HAI-I gene in RCC tissues was negative correlated to the MVD (r ＝ -0.516,P ＜ 0.01 ).Conclusions There is a certain relationship between the missing expression of HAI-1 gene in RCC and the development,invasion and metastasis of RCC.The HAI-1 gene deletion can be used as an important molecular marker in the invasion and metastasis of RCC.