摘要目的 明确绝经前子宫内膜癌组织中微小RNA(MicroRNA)-15b表达水平,并分析其与临床病理参数的关系.方法 收集2016年3月至2018年6月陕西省人民医院妇科经手术切除送检的64例绝经前子宫内膜癌组织及28例绝经前正常子宫内膜组织,应用逆转录多聚酶链反应(RT-PCR)法检测MicroRNA-15b的表达情况,并分析MicroRNA-15b表达水平与绝经前子宫内膜癌病理参数的关系.结果 绝经前子宫内膜癌中MicroRNA-15b的相对表达量低于正常子宫内膜组织(1.38 ± 0.28比2.55 ± 0.36),差异有统计学意义(P<0.05).Ⅲ期、浸润深度≥1/2肌层、发生淋巴结转移、雌激素受体(ER)阴性、孕激素受体(PR)阴性的绝经前子宫内膜癌组织中MicroRNA-15b表达水平显著低于Ⅰ～Ⅱ期、浸润深度＜1/2肌层、无淋巴结转移、ER阳性、PR阳性的癌组织(P＜0.05);而不同年龄、不同病理类型、不同病理分级的绝经前子宫内膜癌组织中MicroRNA-15b表达水平比较差异无统计学意义(P＞0.05).结论 绝经前子宫内膜癌组织中MicroRNA-15b表达水平低,且与病理分期、浸润深度、淋巴结转移及激素受体密切相关,上调MicroRNA-15b表达或可成为治疗绝经前子宫内膜癌新的方向.

abstractsObjective To determine the expression level of MicroRNA-15b in premenopausal endometrial carcinoma and analyze its relationship with pathological parameters. Methods From March 2016 to June 2018, 64 cases of premenopausal endometrial carcinoma tissues and 28 cases of premenopausal normal endometrial tissues that were surgically resected and examined in Shaanxi People′s Hospital were collected. The expression of microrna- 15b was detected by reverse transcription polymerase chain reaction (rt-pcr), and the relationship between the expression level of microrna-15b and the pathological parameters of premenopausal endometrial carcinoma was analyzed. Results The relative expression level of microrna-15b in premenopausal endometrial cancer (1.38 ± 0.28) was lower than that in normal endometrial tissue (2.55 ± 0.36), and the difference was statistically significant (P<0.05). MicroRNA-15b expression level in premenopausal endometrial carcinoma tissue of Ⅲ period, infiltration depth 1/2 or more muscle layer, lymph node metastasis, ER negative and negative PR was significantly lower than that of stage Ⅰ ～ Ⅱ, < 1/2 muscularis infiltration depth, no lymph node metastasis, and positive ER and PR(P < 0.05). However, there was no significant difference in the expression level of microrna-15b in premenopausal endometrial cancer tissues with different ages, pathological types and pathological grades (P>0.05). Conclusions The expression of MicroRNA-15b in premenopausal endometrial carcinoma is low, and it is closely related to pathological stage, depth of invasion, lymph node metastasis and hormone receptor. Up-regulation of microRNA-15b expression may become a new treatment direction for premenopausal endometrial cancer.