摘要Objective: To investigate the association between age and semen parameters among male partners of subfertile couples. Methods: This retrospective study analyzed the semen of 1523 infertile men aged 26 to 50 years. Data were extracted from GarbhaGudi IVF Centre database from January 2019 to September 2020. The basic semen parameters were interpreted according to the WHO manual 2021, 6th edition. Semen parameters in different age groups were compared. Results: Total and progressive motile sperms were significantly higher in the age group of 26-30 years compared to other age groups (P<0.05). Normal sperm count was significantly higher in the age group of 26-30 years compared to the age groups of 41-45 years and >46 years (P=0.001). However, sperm head defects, neck and midpiece defects, tail defects, and cytoplasmic droplets showed statistically insignificant difference in all the age groups (P>0.05). Semen viscosity showed no statistical difference in all the age groups compared to the reference age group of 26 to 30 years. Conclusions: Higher age can lead to a significant decrease in normal sperms and motility in subfertile men. Hence, male partner age should be considered as one of the major determining factors for reproductive outcomes.