摘要Cardiac arrest(CA)is a life-threatening condition with complex pathophysiology and limited treatment options.To gain deeper insights into the pathological state of vital organs,we employed a proteomics analysis in rodents to assess proteome alterations in the brain,heart,kidney,and liver using a rat model of CA.The brain displayed severe protein alterations in essential cellular pathways,including three major energy-generating pathways after CA,which worsened after resuscitation,resulting in the most significant overall protein changes among the organs.Conversely,the liver,experiencing the most substantial protein alterations post-CA,demonstrated significant recovery,presenting the least protein changes post-resuscitation.This remarkable liver recovery may be attributed to the preserved integrity of its energy generation pathways following CA.Our study provides a comprehensive overview of proteome dynamics in critical organs following CA and resuscitation,offering novel insights into the molecular basis for poor brain recovery.The findings emphasize the importance of the brain's energy production capacity in its functional restoration,highlighting the significance of sustaining adequate energy supply in therapeutic approaches.