摘要Antibody drugs,such as monoclonal antibodies and antibody-drug conjugates,have shown significant potential in treating diseases due to their high specificity and affinity.In vivo analysis of antibody drugs with non-invasive and real-time techniques is of importance to understand dynamic behavior of drugs within living organisms,and help evaluate their pharmacokinetics and efficacies.This review summa-rizes the advances and in vivo analysis methods of antibody drugs,including the techniques of radio-labeled imaging,near-infrared fluorescence imaging and surface-enhanced Raman spectroscopy.The principles,applications,and challenges of each technique are discussed,which provides insights for the development of antibody drugs and in vivo analytical methods.