摘要Chemical integrity is indispensable for advancing healthcare by ensuring the availability of high quality,safe,and effective pharmaceutical products.Ingredient quantification is particularly pivotal in this process.Nuclear magnetic resonance(NMR)spectroscopy is a powerful tool for both qualitative and quantitative analysis for complex systems.Compared with 1D quantitative 1H NMR(1H qNMR),quan-titative 13C NMR(13C qNMR)holds some unique advantages.This technique offers a broader chemical shift range and the resulting much lesser signal overlap compare to 1H NMR spectroscopy.This review summarizes relevant studies on the use of 13C qNMR as a quantification technique,along with a focus on quantitative principles,influencing factors,and technical improvements of 13C NMR.The review also highlights its applicability in quantifying diverse molecular structures in pharmaceutical analysis.In addition,potential of low-field NMR,artificial intelligence(AI)-driven method development,and hy-phenation of NMR with other techniques for 13C qNMR analysis is discussed and summarized as well.As a versatile method,13C qNMR holds great potential,and ongoing research is expected to unlock its full capabilities and expand its range of applications.