摘要目的 对急性坏死性胰腺炎(ANP)大鼠离体胰腺组织块行高分辨魔角旋转核磁共振波谱分析(HR-MASNMR),探索其代谢变化特征.方法 按完全随机法将30只Wistar大鼠分为ANP组(20只)和对照组(10只).ANP组大鼠经腹腔分2次注射L-精氨酸2.5 mg/g体重方法 制备,对照组仅注射等容积的生理盐水.运用HR-MASNMR分析两组离体胰腺组织代谢物的含量.结果 造模12 h后,胰腺水肿伴出血、实质内大片凝固性坏死、间质中炎性细胞浸润,并见胰周脂肪皂化;血清淀粉酶水平为(3527±429)U/L,显著高于对照组的(1250±188)U/L.波谱分析显示ANP组胰腺组织的牛磺酸(Tau)、乙酸(Ace)、丙氨酸(Ala)波峰下面积较对照组显著增加(P＜0.05);甜菜碱(Bet)、磷酸胆碱+甘油磷酸胆碱(Pc+Gpc)含量较对照组降低(P＜0.05);胆碱(Cho)、谷氨酸(Glu)、乳酸(Lac)波峰下面积与对照组无明显差异.结论ANP大鼠离体胰腺组织块具有显著的代谢特征,为进一步开展人类重症急性胰腺炎在体波谱研究奠定了实验基础.

abstractsObjective To investigate the metabolite features of acute necrotizing pancreatitis in rats in vitro by high resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MASNMR).Methods A total of 30 Wistar rats were randomized into ANP group ( n = 20) and control group ( n = 10).All the rats in ANP group were injected with L-arginine 2.5mg/g body weight twice, and the animals in the control group received same dose of saline. HR MASNMR was used to study the metabolic changes of acute necrotizing pancreatitis in rats in vitro. Results 12 hours after the ANP induction, the pancreas were more swelling, presented with bleeding points, with mild increase in liquefied change, coagulation necrosis could be found in parenchyma and a large number of fatty tissues could be seen around the pancreas. Serum amylase level was ( 3527 ± 429 ) U/L, which was significantly higher than ( 1250 ± 188 ) U/L in control group.Compared with those in the control group, the signal intensity of taurine ( Tau), acetic acid ( Ace), alanine (Ala) of the ANP group were significantly increased. While the signal intensities of phosphocholine (Pc),glycerophosphocholine (GPc) and betine (Bet) were significantly decreased. The signal intensities of choline (Cho), glutamic acid (Glu), lactate (Lac) were not significantly different. Conclusions There were obvious metabolic features of pancreatic tissues of ANP in rats, and it is useful for the application of magnetic resonance spectroscopy in AP in vivo in human studies.