摘要The basidiomycetes Stereum hirsutum and Boreostereum vibrans are two fungi of the same genus.In this study,chemical investigation on the co-cultures of the two congeneric fungi led to the isolation of eleven new vibralac-tone derivatives,hirsutavibrins A-K(1-11).The structures of 1-11 were elucidated by extensive NMR and HRESIMS spectroscopic analysis,and computational methods.Hirsutavibrins A(1)and B(2)showed weak cytotoxicity against the human lung cancer cell line A549.Hirsutavibrin D(4)showed moderate anti-nitric oxide activity in murine monocytic RAW 264.7 macrophages.This work not only expands the members of vibralactone derivatives with variable configurations but also opens a new avenue for fungal co-culturing study between congeneric fungi.