摘要Sesquiterpenoids represent a structurally diverse class of natural products widely recognized for their ecological significance and pharmacological potential,including antimicrobial,anti-inflammatory,and anticancer properties.As part of our efforts to explore bioactive secondary metabolites from phytopathogenic fungi,we conducted a molecu-lar networking-based analysis of Bipolaris sorokiniana isolate BS11134,which was fermented on a rice medium.This analysis led to the identification of three new seco-sativene-type sesquiterpenoids(1-3)and seven known analogues(4-10),with the NMR data of compound 4 being reported for the first time.The structures of these compounds were elucidated using HR-ESI-MS and extensive spectroscopic data analysis.Notably,compound 9 significantly inhib-ited nitrous oxide expression in lipopolysaccharide(LPS)-treated RAW264.7 cells in vitro(inhibition rate:84.7±1.7％at 10 μM),while compound 1(10 μM)showed a weak inhibitory effect(inhibition rate=28.0±2.4％).Additionally,we proposed a biosynthetic pathway for these compounds.This study not only expands the chemical space of the hel-minthoporene class of molecules but also underscores the untapped potential of phytopathogenic fungi as promis-ing sources of structurally unique and biologically active natural products.