摘要Based on two mitochondrial genes (cyt b,ND4) and one nuclear gene (c-mos),we explored the relationships within the Asian pit viper genus Gloydius.In total,23 samples representing 10 species were analyzed.All phylogenetic analyses support a monophyletic Gloydius with two major clades,one comprising G.brevicaudus,G.blomhoffii,and G.ussuriensis with the sister clade consisting of G.intermedius,G.saxatilis,G.halys and G.shedaoensis.The relationships among the three montane species G.strauchi,G.qinlingensis and G.liupanensis,as well as the two monophyletic groups,are unstable,and discussed.Divergence date estimation indicates that Gloydius lineage formed 15 Ma and diversification of the genus occurred at 9.89 Ma.Issues regarding the taxonomy of this genus are discussed where necessary.