摘要We described a new species, Microhyla beilunensis sp. nov., from Zhejiang Province of China. Phylogenetic analyses based on the mitochondrial 12S, 16S and CO1 gene sequences suggested that the new taxon was distinctly separated from its congeners and closed to M. mixtura and M. okinavensis. Morphologically, the new species could be identified from its congeners except M. mixtura by several characters: (1) rudimentary webs on toe base; (2) absence of disks and dorsal median longitudinal grooves on finger tips; (3) presence of disks and dorsal median longitudinal grooves on toe tips. As well, the new species could be identified from topotype M. mixtura by the combination of characters: (1) apart from the stripes, bar-shaped and oval-shaped patterns, the rounded spots present on the dorsum of body and legs; (2) the outer metacarpal tubercles prominently larger than the inner one; (3) of males, the ratios of HW, IND, UEW and LAW to SVL of the new species were significantly larger than those of M. mixtura (P < 0.01), and the ratios of SL, IOD, LAHL, HLL, TL, TFL and FL to SVL of the new species were significantly less than those of M. mixtura (P < 0.05).