摘要As an organophosphorus compound that frequently detected in water samples, triphenyl phosphate (TPhP) has been showed to have multiple toxicological effects on aquatic species. However, no attention has been paid to its potential impact on non-model amphibian species. Here, tadpoles of the Zhenhai brown frog (Rana zhenhaiensis) were exposed to different concentrations of TPhP (0, 0.02 and 0.1 mg/L) throughout the developmental period to assess physiological and metabolic impacts of TPhP exposure on amphibian larvae. After 30-day TPhP exposure, the developmental stage of tadpoles from the high-concentration treatment appeared to be more advanced than that from the other two treatments, but other measured traits (including body size, tail length and liver weight) did not differ among treatments. Metabolite profiles in tadpole livers based on liquid chromatography-mass spectrometry (LC-MS) revealed a distinct metabolic disorder in exposed animals. Specifically, significant changes in various hepatic amino acids (such as glutamine, glutamate, valine and leucine) were observed. Overall, our results indicated that chronic TPhP exposure potentially caused developmental and hepatic physiological changes in R. zhenhaiensis tadpoles, although its impact on tadpole growth appeared to be minor.