摘要Advertisement calls are essential for communication among anuran amphibians,with their production influenced by a range of environmental factors and morphological traits.To understand the primary sources of variation of advertisement calls in male Leptobrachium liui,we recorded their advertisement calls during the breeding season and analyzed two primary acoustic parameters:call duration(CD)and dominant frequency(DF),along with their within-individual coefficients of variation.Additionally,we collected nest-related environmental parameters and morphological traits to investigate how these factors influence the variation in advertisement calls.Our analyses revealed that both nest-related environmental parameters(particularly water temperature)and individual morphological traits(especially head morphology)significantly influenced advertisement call characteristics in male L.liui.Water temperature showed a negative relationship with CD,while head morphology was positively associated with both CD and DF.These findings enhance our understanding of the factors shaping acoustic communication in L.liui during the breeding season and highlight the importance of both environmental and morphological factors in anuran vocalization.