摘要Recently,the highly ordered J-aggregates of organic dyes with intriguing optical properties have received considerable attention in biomedical applications.Herein,binary metal ions Mn(Ⅱ)/Fe(Ⅲ)are used to induce the formation of indocyanine green(ICG)J-aggregates.Further,the sheet-like J-aggregates are able to act as"carriers"for loading hydrophobic chemotherapeutic gambogic acid(GA),realizing the effect of"killing two birds with one stone"for both treatment and delivery.The as-designed nanoassembly is formed spontaneously in aqueous environment via π-π stacking,electrostatic interaction,and hydrophobic force,exhibiting enhanced photostability of ICG and outstanding reactive oxygen species(ROS)generation ability.Moreover,significant inhibition of tumor growth by the synergetic effect of phototherapy and chemotherapy is verified in a subcutaneous 4T1 tumors model.In conclusion,this work not only presents a facile and green approach to manufacture carrier-free nanodrugs,but also establishes a universal platform that has potential application in the co-delivery of near-infrared dye and hydrophobic molecules.