Genome sizes of four important medicinal species in Kadsura by flow cytometry

导出学术成果认领

摘要Objective:Dianjixueteng is a geoherb in Yunnan Province,the source plant of which is Kadsura interior.However,the formation of this geoherb is not clear in genetic mechanism,in which genome size is the first step that should be known on the genomic level.In this study we aimed to estimate the genome sizes of source plants of K.interior and three related herbs K.heteroclita,K.longipedunculata,and K.coccinea by flow cytometry(FCM)and make a comparison.Methods:The genome sizes of K.interior,K.heteroclita,K.longipedunculata and K.coccinea,i.e.,the source plants of Dianjixueteng and its relative medicinal materials,were estimated by FCM.The nuclei of K.inte-rior were isolated using modified LB01 buffer,for the rest species,by the Galbraith's buffer.Results:The genome sizes of K.interior,K.heteroclita,K.longipedunculata,and K.coccinea were 7.36,7.12,7.01,and 5.15 pg/1C,respectively.Genome size of K.interior had no significant variation with those of K.heteroclita and K.longipedunculata(P = 0.296),which was significantly larger than that of K.coccinea.Conclusion:Genome size can not distinguish K.interior from K.heteroclita and K.longipedunculata,but could distinguish them from K.coccinea,which lays the foundation for future studies on genetic mecha-nism of the geoherb formation.

作者 Jing Xu ^[1] Xue-ping Wei ^[2] Jiu-shi Liu ^[2] Yao-dong Qi ^[2] Ben-gang Zhang ^[2] Hai-tao Liu ^[2] Pei-gen Xiao ^[2] 学术成果认领

作者单位 Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China ^[1] Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China;Engineering Research Center of Tradition Chinese Medicine Resource,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China ^[2]

关键词 Dianjixueteng Flow cytometry Genome size Kadsura interior A.C.Smith

栏目名称 Original Articles

发布时间 2021-10-11

基金项目

We sincerely thank Mr Zhi-liang Peng(Guihuashu Forest Farm of Fengqing Forestry Bureau)and Mr Liang-yi Yang(Guihuashu Forest Farm of Fengqing Forestry Bureau)for collecting organisms of Kadsura species We also thank Prof Xian-chun Zhang(1BCAS,The Institute of Botany,Chinese Academy of Sciences) and Miss Si-qi Liang(IBCAS)for providing organisms of reference standards plants We thank Mrs Jin-dan Zhang from the Plant Science Facility of IBCAS for her excellent technical assistance on FCM This study was financially supported by the National Natural Science Founda-tion of China

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