摘要Objective:Acute liver injury(ALF)is a potential factor of many serious hepatopathies.Carbon tetrachlo-ride(CCl4)is a possible environmental toxicant that can induce ALF.Portulaca oleracea(PO)is one of the most popular edible herbs and has several biological activities such as antioxidant,antimicrobial,anti-inflammatory effects.We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods:The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo.Hepatic levels of transaminase activities and inflammatory factors were examined.The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis.Meanwhile,the efficacy of PO was certified by HepG2 cells in vitro.The transaminase activities,inflammatory factors,and the pro-tein expression of S100A8 and S100A9 were also detected.Results:Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT,AST,ALT and LDH,as well as reducing the pro-inflammatory cytokines(IL-1β,IL-6,TNF-α)secretion in CCl4-induced liver injury mice.Simultaneously,HepG2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST.Moreover,PO resulted in a sig-nificant downregulation of the pro-inflammatory markers S100A8,S100A9 gene and protein expression on CC14 induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion:PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines'release,indicating a potential clinical effect for controlling the disease.