摘要Objective:Secondary metabolites and polyphenolic compounds from medicinal plants have been demon-strated to have multiple biological functions with promising research and development prospects.This study examined the effect of β-stigmasterol(with ergosterol)and xylopic acid isolated from Anchomanes difformis on liver mitochondrial permeability transition pore(mPTP).Methods:The compounds were isolated by vacuum liquid chromatography.Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions.Results:1H and 13C NMR spectroscopic elucidation of the isolates affirmed the presence of β-stigmasterol with ergosterol(1∶0.3)and xylopic acid.The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose.Pharmacological inhibition of mPTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders.Conclusion:Reduction in the activity of calcium ATPase and the expression of Caspase-3 and-9 were observed,suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.