摘要Objective:To study the correlation between hyaluronic acid(HA),hyaluronic acid synthase(HAS)and human renal clear cell carcinoma(RCCC).Methods:The expression of three HAS isoforms' gene and HA in 93 RCCC tissues,27 nephridial tissues by the side of RCCC from two hospitals were measured with Real-Time RT PCR.Western Blot and immunohistochemical methods and analyzed.Results:All RCCC and adjacent normal tissues expressed three HASs' mRNA & protein;at the mRNA level,both RCCC and adjacent normal tissues,expressed more HAS3 than HAS1 or HAS2,their differences were statistically significant(all P values <0.05);but,at the protein level,all HAS isoforms presented the equivalent expression.Compared with the adjacent non-neoplastic kidney tissues,the expression of all HAS isoforms' mRNA in RCCC tissues were increased evidently and their differences were significant(all P values <0.0001);but at the protein level,only the expression of HAS3 increased evidently(P=0.022).In all adjacent normal tissues,more than 80%renal tubular cells strongly expressed HA,however,only the minority RCCC cases(16/93)presented weakly positive HA staining in few cancer nests(5%-30%),the difference were significant(P<0.0001).In RCCC tissues subgrouped according to clinical stage,pathological grade,lymphatic metastasis or not and distant metastasis or not,the HAS mRNA & protein differential expression all had no statistical significance(all P values >0.05).Conclusion:Different from other malignancy,HA and HASs(except for HAS3)may not play important roles in the biological progress of human RCCC.