摘要Viruses in aquatic ecosystems are characterized by extraordinary abundance and diversity.Thus far,there have been limited studies focused on viral communities in river water systems.Here,we investigated the virome of the Yangtze River Delta using viral metagenomic analysis.The compositions of viral communities from six sampling sites were analyzed and compared.By using library construction and next generation sequencing,contigs and singlet reads similar to viral sequences were classified into 17 viral families,including nine dsDNA viral families,four ssDNA viral families and four RNA viral families.Statistical analysis using Friedman test suggested that there was no significant difference among the six sampling sites(P＞0.05).The viromes in this study were all dominated by the order Caudovirales,and a group of Freshwater phage uvFW species were particularly prevalent among all the samples.The virome from Nanjing presented a unique pattern of viral community composition with a relatively high abundance of family Parvoviridae.Phylogenetic analyses based on virus hallmark genes showed that the Caudovirales order and CRESS-DNA viruses presented high genetic diversity,while viruses in the Microviridae and Parvoviridae families and the Riboviria realm were relatively conservative.Our study provides the first insight into viral community composition in large river ecosystem,revealing the diversity and stability of river water virome,contributing to the proper utilization of freshwater resource.