摘要Human metapneumovirus(HMPV)infection is one of the leading causes of hospitalization in young children with acute respiratory illness.In this study,we prospectively collected respiratory tract samples from children who were hospitalized with acute lower respiratory tract infection in six hospitals in China from 2017 to 2019.HMPV was detected in 145 out of 2733 samples(5.3％)from the hospitalized children.The majority of HMPV-positive children were under the age of two(67.6％),with a median age of one year.HMPV can independently cause acute lower respiratory tract infection in young children,while all patients showed mild clinical symptoms.Of all the co-infected patients,HMPV was most commonly detected with enterovirus(EV)or rhinovirus(RhV)(38.0％,followed by respiratory syncytial virus(RSV)(32.0％).The highest detection rate occurred from March to May in both northern and southern China.Out of 145 HMPV positive samples,48 were successfully typed,of which 36 strains were subgrouped into subtypes A2c(75％),eight strains were included in subtype B1(16.7％),and four strains were included in subtype B2(8.3％).Moreover,16 A2c strains contained 111-nucleotide duplications in the G gene.Twenty-seven complete HMPV genomes were successfully obtained,and 25(92.6％)strains belonged to subtype A2c,whereas one strain was included in subgroup B1 and another was included in subgroup B2.A total of 277 mutations were observed in the complete genomes of 25 A2c strains.All results presented here improve our understanding of clinical characteristics and molecular epidemiology of HMPV infection in children.