摘要Noroviruses(NoVs)are the most significant viral pathogens associated with waterborne and foodborne outbreaks of nonbacterial acute gastroenteritis in humans worldwide.This study aimed to investigate the prevalence and diversity of NoVs contaminated in the environmental water in Chiang Mai,Thailand.A total of 600 environmental water samples were collected from ten sampling sites in Chiang Mai from July 2020 to December 2022.The presence of NoV genogroups I(GI),GII,and GIV were examined using real-time RT-PCR assay.The genotype of the virus was determined by nucleotide sequencing and phylogenetic analysis.The results showed that NoV GI and GII were detected at 8.5%(51/600)and 11.7%(70/600)of the samples tested,respectively.However,NoV GIV was not detected in this study.NoV circulated throughout the year,with a higher detection rate during the winter season.Six NoV GI genotypes(GI.1-GI.6)and eight NoV GII genotypes(GII.2,GII.3,GII.7,GII.8,GII.10,GII.13,GII.17,and GII.21)were identified.Among 121 NoV strains detected,GII.17 was the most predominant genotype(24.8%,30 strains),followed by GII.2(21.5%,26 strains),GI.3(17.4%,21 strains),and GI.4(16.5%,20 strains).Notably,NoV GII.3,GII.7,GII.8,and GII.10 were detected for the first time in water samples in this area.This study provides insight into the occurrence and seasonal pattern of NoV along with novel findings of NoV strains in environmental water in Thailand during the COVID-19 outbreak.Our findings emphasize the impor-tance of further surveillance studies to monitor viral contamination in environmental water.