摘要Mammals are critical reservoirs of human infectious diseases and the spillover of viruses is related to climate conditions.We conducted meta-transcriptomic sequencing of 226 mammals(bats,rodents,hedgehogs,and shrews)representing 20 species collected across eight cities in south China between 2018 and 2024.Samples included internal organs,oropharyngeal and anal swabs,and feces.We identified 63 vertebrate-associated viruses,including 34 novel viruses.Phylogenetic analysis revealed six viruses with potential infection risks to humans or domestic animals due to their close phyloge-netic relationships with known pathogens.Cross-species transmission was observed in 14.3%(9/63)of viruses,shared by at least two host species,with bats,particularly Rhinolophus and Hipposideros,serving as key hubs for viral circulation and zoonotic spillover.Virome composition varied sub-stantially among mammalian species and geographic regions(adonis test,R2=0.50,P=0.001).Generalized linear models quantified the roles of host taxonomy,ecotypes,and meteorological factors in shaping viral diversity,demonstrating host taxonomy(at the order level)as a predominant role(25.70%deviance explained),followed by ecotypes(10.27%deviance explained).Phylogenetic analysis conducted using our betacoronavirus sequences,as well as betacoronavirus sequences derived from 2.0×104 bats sampled in China between July 2013 and March 2024,revealed that no betacoronaviruses exhibited closer phylogenetic relationships to SARS-CoV-2 than the known strains(e.g.,RaTG13).These findings provide critical insights into virus evolution,transmission,and ecological determinants,which are essential for the prevention of emerging infectious diseases.